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Supporting
laboratory methods

The goal of our treatment is a successful pregnancy leading to the birth of a healthy baby. That's why we use state-of-art technologies that increase the chance of conception. At Unica clinics, we offer you a wide range of these support methods. Based on the examination results, our doctors and embryologists will advise you on which of the listed methods is the most suitable.

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Functional Sperm Selection (PICSI)

In this technique, we use the fact that the eggshell contains hyaluronan and the heads of mature sperm have a developed receptor for this substance. We use hyaluronan as a magnet to attract mature sperm. Thanks to this method, we can preselect suitable sperm and fertilize the egg with them. Unlike the ICSI method, where we select sperm by shape and motility, PICSI allows us to select mature sperm. This method is thus very similar to selection during natural conception.

Who is PICSI method suitable for?

• patients with borderline spermiogram values

• patients after several unsuccessful cycles

• couples with low fertilization or poor embryo quality

Magnetically Activated Cell Sorting (MACS)

This non-invasive sperm selection method allows us to select live sperm without signs of apoptosis. Apoptosis is the process of programmed cell death. At first, spermatozoa may appear mobile; under a microscope, it is difficult to distinguish them from healthy sperm. However, apoptotic sperm cannot fertilize the egg at a later stage. The MACS method magnetically marks these sperm and captures them using the magnetic field. The remaining sperm passing the method show higher mobility, shape and DNA fragmentation.

Who is the MACS method suitable for?

• Men at an older age due to the probability of having a DNA-damaged sperm

MicroChip Fertile Plus® (Microfluidic sperm sorting chips)

Some assisted reproduction procedures require so-called centrifugation in the preparatory process. It mechanically filters out unsuitable sperm. But at the same time, it means higher stress, which often leads to sperm damage. The microfluidic chip method is a more gentle alternative based on the principle of natural sperm selection. The chip contains micro-barriers that the sperm pass through, which mimic the natural environment of a woman's fallopian tubes. They can be used for fertilization by ICSI, PICSI, IVF, and IUI methods. Sperm sorted in this way show higher mobility and better shape, lower DNA fragmentation and levels of reactive oxygen species, which could negatively affect them.

Who is the MicroChip Fertile Plus® method suitable for?

• for couples after repeated miscarriages

• for couples after repeated embryo transfers, and embryo implantation failures

• for couples with poor embryo quality in previous cycles

Timelapse

This method helps us monitor embryo development throughout its cultivation. We place the embryos in a special Timelapse culture box, where they have the best conditions for growth. We continuously scan them with a microscopic camera and monitor their development. During the entire 5 days of extended cultivation, we evaluate the development dynamics. Subsequently, we can select the best quality embryos with the best chance of pregnancy.

Assisted hatching using a laser

Each egg and later the embryo is located in a protective covering called the zona pellucida. Here it remains until the implantation time in the uterus. Some embryos, however, have a significantly strong shell, which prevents them from successfully nesting. In the assisted hatching technique, we use a laser beam to create a hole in the embryo's protective cover. This way, we ease the contact with the uterus cells and support the implantation and thus a successful pregnancy. Assisted hatching using a non-contact laser is significantly gentler on the embryo than mechanical hatching and, therefore, cannot damage it.

EmbryoGlue®

Médium od společnosti Vitrolife, EmbryoGlue®, je speciální tkáňové lepidlo, které využíváme při transferu embrya do dělohy. Obsahuje vyšší koncentraci látky hyaluronan, která se běžně vyskytuje ve vejcovodech a děloze a vytváří prostředí se zvýšenou viskozitou. Embryo vložené do dělohy na speciálním médiu EmbryoGlue® se tak jednoduše řečeno přilepí na děložní stěnu a má větší šanci na uchycení. Klinické výsledky ukazují, že použitím média EmbryoGlue® vzrůstá četnost těhotenství pacientek o více jak 6 %. Kultivační médium EmbryoGlue® lze použít při embryotransferu všech vývojových stádií embryí, od dvoudenních až po blastocystu. Je vhodné pro čerstvá i zmrazená embrya.

Aspiration of sperm from the epididymis (MESA)

If there is no sperm in the man's ejaculate, we choose the epididymal sperm aspiration method (MESA). This microsurgery is performed by a doctor specializing in urology while the patient is under full anaesthesia. The doctor gains access to the epididymis through an external cut on the scrotum, where he retrieves the sperm with a pipette. The cells collected in this way can be used for fertilization using the ICSI method when sperm is injected directly into the egg.

Who is the MESA method suitable for?

• for men with impaired sperm transport between the epididymis and the urethra

• for men after vasectomy

Retrieval of sperm from testicular tissue (TESE)

If there is no sperm in the ejaculate or epididymis, we obtain cells from testicular tissue (TESE). A urologist performs this microsurgery under general anaesthesia. He removes small pieces of testicular tissue through an external cut on the scrotum. From there, the sperm itself is subsequently obtained in the laboratory. The cells obtained in this way can then be used for fertilization by the ICSI method (injection directly into the egg) and the MESA method.

Who is the TESE method suitable for?

• for men with impaired sperm transport between the epididymis and the urethra

for men after vasectomy

ERA (Endometrial Receptivity Analysis)

The endometrial receptivity test determines when the endometrium is in optimal condition for successful embryo implantation. Based on that, we plan a transfer for the time with the highest chance of pregnancy.

Who is the ERA method suitable for?

• couples who repeatedly experience failure to implant an embryo

EndomTRIO (EMMA, ERA, ALICE)

This set of three examinations, EMMA, ERA and ALICE, gives us a comprehensive view of the endometrium state. Thanks to these tests, doctors recommend an appropriate course of treatment and thus increase the chance of a healthy pregnancy.

EMMA (Endometrial Microbiome Metagenomic Analysis)

This test analyzes the microbiological balance of the endometrium. It evaluates the presence of bacteria, including those supporting a successful pregnancy.

ERA (Endometrial Receptivity Analysis)

This examination determines when the endometrium is in optimal condition for successful embryo implantation. Based on that, we plan a transfer for the time with the highest chance of pregnancy.

ALICE (Analysis of Infectious Chronic Endometritis)

This examination is part of the EMMA test and can identify chronic endometrial inflammation and pathogenic bacteria, most often associated with chronic endometritis. It can be one of the causes of embryo implantation disorders or repeated miscarriages.

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Related treatments

Assistierte Reproduktion

Wir bieten eine breite Auswahl von Methoden und Verfahren an, dank denen auch unfruchtbare Paare Eltern werden können. Individuelle Lösungen und Betreuung sind bei Unica selbstverständlich.

Holistic fertility

Unica ist die einzige in der Tschechischen Republik, die eine einzigartige Kombination von erstklassiger IVF-Behandlung und Ganzheitliche Medizin anbietet. Unterstützen Sie die Empfängnis mit Akupunktur, Yoga oder Physiotherapie.

Diagnostik und Fertilitäts Unterstützung

Dank einer umfassenden Kinderwunschuntersuchung erfahren Sie, wie Ihre Chancen stehen, Eltern zu werden. Wir werden die geeigneten Maßnahmen oder medizinischen Verfahren empfehlen

Genetik

Erweiterte Gentests prüfen, ob Sie nicht Träger von genetischen Mutationen sind, die die Schwangerschaft oder die Gesundheit des zukünftigen Kindes gefährden.

Gynäkologie

Besuchen Sie hochmoderne gynäkologische Praxis im Umfeld einer Luxusklinik. Sie können ohne Wartezeit online einen Termin vereinbaren.

We’re here for you

Any further questions? Our coordinator will answer all your questions and arrange everything for your upcoming visit to our clinic.

Katerina-photo
Katerina

clinic Prague

+420 736 689 105

Helena-photo
Helena

clinic Brno

+420 735 759 071

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